Coding

Part:BBa_K4229023:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-28)


sfGFP with AMBER stop codon at the position of the 74th amino acid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Was taken from the iGem Team Freiburg 2019. GFP is a fluorescent protein taken from Aequorea victoria, which was then improved into the superfolder GFP form(sfGFP).

References

H. Chen, J. Wilson, S. Ottinger, Q. Gan, and C. Fan, “Introducing noncanonical amino acids for studying and engineering bacterial microcompartments,” Curr. Opin. Microbiol., vol. 61, pp. 67–72, 2021, doi: 10.1016/j.mib.2021.03.004.

Q. Gan, B. P. Lehman, T. A. Bobik, and C. Fan, “Expanding the genetic code of Salmonella with non-canonical amino acids,” Sci. Rep., vol. 6, no. November, pp. 1–7, 2016, doi: 10.1038/srep39920.